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All University of South Carolina system institutions will be closed through the end of the spring semester. Columbia campus virtual instruction will continue through the end of the Summer II semester (Aug 1). Details can be found on the coronavirus landing page.

School of Medicine

Services and Fees

Order Forms Download: [PDF Version] - [Word Version]

Shipping for all vectors is $150.00
Prep  Vector
titer*
Volume
Provided
Prep Concentration Gold
Titering*
RCR
assay*
USC External Academic Corporate
Small  1x10E7
TU/mL
0.15 ML  -  +  - $300 $500 $700
Medium   1x10E9
TU/mL
0.3 ML  +  +  + $500 $750 $1000
Large  1X10E9
TU/mL
0.6 ML  +  +  + $1000 $1500 $1700

Vector titer*-guaranteed minimum of 1x10E7 TU/ml; usually 1x10E7-1x10E9 TU/ml

Gold Titering*-titers will be measured by three different assays: P24 ELISA, real-time PCR, targeted cells counting if reporter transgene*

RCR assay *- mandatory for the concentrated preps and optional for the non-concentrated (small) 
prep. Extra charge of $50 applied

Lentivirus is supplied in 10 μl aliquots unless otherwise specified.

Approximate turnaround time is two weeks for the non-concentrated vectors and four to six weeks for the concentrated vectors

Prep  Vector
titer*
Volume
Provided
Prep Concentration Gold
Titering*
USC External AcademicC Corporate
Prep     Vector
titer*
Volume
Provided
Prep Concentration Gold Titering* USC External Academic Corporate
 Small 1x10E11
vg/mL
150 µL + + $200 $300 $500
 Standard 1x10E12
vg/ml
0.5 mL + + $500 $750 $1000

Vector titer*-guaranteed minimal; usually higher

Gold Titering*-titers will be measured by two different assays: real-time PCR, targeted cells counting if reporter transgene included

Approximate turnaround time is two to three weeks for the Standard Prep

The viral core is no longer providing HSV-based viral vectors.

  *Not all cloning services are currently available at this time.  Please consult with Christopher Seliga (Christopher.Seliga@uscmed.sc.edu) before requesting cloning services
Service   Description Cost
Cloning* Subcloning of cDNA into vector cassette $850
Cloning* Single-step cloning -- additional plasmid $600
Cloning* Multiple step/large fragments (>10kb)/insert by 
PCR/Genetic mutagenesis
$1,250
Plasmid Prep Plasmid amplification-Maxi Prep $200
p24 ELISA p24 ELISA/Plate $500
Comp cells HB101 1ml (20x50ul)>108cfu/µg $150
Cesium Prep Cesium Grade DNA 1mg $750

 

 


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