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School of Medicine


Instrumentation Resource Facility

The mission of the IRF is to provide state-of-the-art biomedical research instrumentation for use in the USC SOM and USC main campus research environments. As time and resources permit, outside use of the facility also is permitted and encouraged. 

State-of-the-art Biomedical Research Equipment

The Instumentation Resource Facility (IRF) is an integral component of the research and teaching mission of the University of South Carolina School of Medicine.

Located within the facility are several major pieces of state-of-the-art biomedical research equipment that provide techniques ranging from whole animal through single cell imaging to analysis at the molecular level. 

The IRF also houses a full range of ancillary equipment that is available for sample preparation. In addition to serving as a resource for acquisition of primary data, the IRF also has the capacity for image enhancement and related data analysis.

Service areas include:

  • Confocal Imaging
  • Electron Microscopy
  • Histology
  • Instrument Training
  • Flow Cytometry/ Cell Sorting
  • Poster Printing 
  • Bio-Rad Ordering 

    The IRF places a weekly order to BioRad. Internal USC customers may submit a product order through the IRF to receive the School of Medicine discount. Weekly order deadline is 12pm Mondays. Orders submitted to the core after the deadline will be processed the following week. All orders should be placed through iLab

iLab Portal

The Instrumentation Resource Facility uses Agilient CrossLab's iLab software to manage equipment availability and service requests. To get started on iLab:

  • Register for an account if you're a first time user.
  • Log in with your iLab credentials in the upper right corner and go to the Request Services tab to submit a service request.
  • Visit the Customer Help Manual for further guidance using iLab's Core Facility Services.


Our Equipment 

Whole Animal Imaging Instruments

Caliper/Xenogen IVIS Spectrum Fluorescence and Bioluminescence Imaging System

Prior to use all researchers must complete a radiation saftey course. After completion of the radiation safety course individuals should contact Dr. Robert Price to set up a training time and to obtain security clearance via a palm reader for access to the unit.

The IVIS Spectrum is the newest instrument in the IVIS line of products and performs bioluminescent and epi- and trans- illumination fluorescent imaging. Unique to the IVIS Spectrum in this line of instruments is the capability to sensitively image both bioluminescent and fluorescent  reporters within the same animal and to provide a spectral fingerprint of the signal produced. This is an important advance in in vivo imaging technology as it allows separation of autofluorescence from the emitted signal improving signal resolution.  To obtain the spectral fingerprint the Spectrum uses a series of high efficiency narrow band-pass filters coupled with spectral unmixing algorithms to differentiate between multiple shallow and deep fluorescent sources. This provides a mechanism to optimally perform the wide array of fluorescence/bioluminescence research applications of our investigators. The system is also capable of performing 3D tomographic reconstructions for both bioluminescence and fluorescence.

  • High-sensitivity in vivo imaging of fluorescence and bioluminescence
  • High throughput (5 mice) with 23 cm field of view
  • High resolution (to 20 microns) with 3.9 cm field of view
  • Twenty eight high efficiency filters spanning 430 – 850 nm
  • Supports spectral unmixing applications which is ideal for distinguishing multiple bioluminescent and fluorescent reporters
  • Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination
  • 3D diffuse tomographic reconstruction for both fluorescence and bioluminescence
  • Ability import and automatically co-register CT or MRI images yielding a functional and anatomical context
  • NIST traceable absolute calibrations
  • Gas anesthesia inlet and outlet ports.
  • Both mouse and rat platforms designed to maintain body temperature during anesthesia
  • Compact isoflurane anesthesiology system with an oxygen regulator and flow meter

Applications

Useful in a wide range of biomedical applications including oncology, cardiovascular diseases, stem cell biology, neurobiology, inflammation, toxicology and drug metabolism


Location
The IVIS Spectrum is located in Building 9  

IRF Contact
Lorain Junor

Website 

 
JL Shepherd and Associates Model 143-68 Blood Product Irradiator 
Prior to use all researchers must complete a radiation safety course with the university.  After completion of the radiation safety course individuals should contact Dr. Robert Price to set up a training time and to obtain security clearance via a palm reader for access to the unit.

The Model 143 Cesium-137 gamma irradiator is a self-contained fully shielded unit capable of delivering a dose rate of up to 200 Roentgen/minute. Final sample dose is determined by duration of irradiation that can be controlled by a preset timer on the unit.

Applications
Irradiation of molecules, cells, tissues, organs or whole animals.

 
Mauna Kea Cellvizio In Vivo Confocal 
The Cellvizio is a dual band (488 and 660nm) probe based confocal laser endomicroscopy system.  It is ideal for molecular imaging at the cell level for preclinical research.  Applications include in vivo imaging in neurosciences (deep brain imaging), cancer and inflammation (spleen, liver, etc) and cardiovascular (vessels, plaques).  Imaging is performed by inserting a small fiber optic probe into an incision. 

Probes

  • 300mm beveled or flat tip (deep brain imaging in mice or other organs if low invasiveness is required)
  • 650mm flat tip (deep brain imaging in rats or other organs if low invasiveness is required)
  • 1.5mm round tip (general applications)
  • 4.2mm high resolution 100nm working distance (vessels and angiogenesis)
  • 1.8mm long working distance (blood flow through vessel wall without penetration, deep cells in tumors or organs)
  • UltraMinio high resolution (vessels, angiogenesis, cell morphology)
  • MiniZ (cavities, eyes)
  • Cerboplex (brain imaging)

Software

Hyperscan Module for ultra-fast imaging
Vessel detection software for automatic delineation of vessels, capillaries and vascular bed
Mosaicing software for stitching images for reconstruction of tissues and organs.

Location
The Cellvizio is located in the Building 9 Small Animal Imaging Suite.

IRF Contact
Bob Price or Lorain Junor

Website


Ultrasound Workstation 

 
Visual Sonics VEVO 3100 High Resolution In Vivo Imaging System 
The VEVO 3100 is an ultra-high frequency ultrasound imaging system for acquisition of longitudinal in vivo anatomical, functional, physiological and molecular data simultaneously in real time at a resolution of 30 mm.  Both mouse and rat platforms designed to maintain body temperature during anesthesia and a compact isoflurane anesthesiology system with an oxygen regulator and flow meter are available.

Software packages

  • Analytical software for B-mode image capture and analysis, ECG on screen tracing, measurements and annotations, cineloop image review capture and display
  • M-Mode capture and analysis software for characterization of vessel and heart dynamics including wall thickness
  • Pulsed Wave Doppler package enabling quantitative analysis of blood flow velocity and waveform measurements
  • EKG and Advanced Cardiovascular
  • Software Packages
  • Contrast Enhancement and microbubble imaging software package
  • Microinjection capability


Scanheads

  • 25MHz/Focal length of 17.3mm/Field of view of 22mm – Imaging deep tissues in larger rats
  • 37.5MHz/Focal length of 15mm/Field of view of 20.8mm – Rat cardiology, EKV, General Imaging
  • 50MHz/ Focal length of 12.7mm/Field of view of 20mm – Adult mouse cardiology
  • 20mm – Adult mouse cardiology
    82.5MHz/Focal length of 4.5mm/Field of view of 17.4mm – Surface imaging (epidermis, skin tumors, bowel imaging, peritoneum

Applications

  • Cardiovascular- enables real time imaging and analysis of physiological function of the heart chambers and associated major vessels and the ability to monitor morphological changes during heart development and pathology.
  • Cancer biology- enables 2D and 3D tracking of the shape and size of subcutaneous and orthotopic tumor growth in longitudinal studies of the same animal during therapeutic treatment. Software allows volumetric analysis of tumor size and tracking of tumor vasculature and blood flow.
  • Neurobiology- enables real time monitoring of embryonic or neonatal brain development prior to ossification of the skull, cell lineage studies, identification of progressive neural degenerative diseases, and visualization of image guided needle injections and extractions.
  • Developmental biology- longitudinal and dimensional assessment of the same animal from early in development through adulthood, analysis of timed pregnant animals for developmental stage.

Location
The VEVO 3100 is located in Building 9 Small Imaging Suite.

IRF Contact
Lorain Junor

Website 

Histology Equipment

Dako Autostainer 
The Dako Autostainer plus system for robotic handling of immunohistochemistry staining
 
Reichert-Jung 2030 Paraffin Rotary Microtomes 


Vibratome Tissue Slicer 


Zeiss Microm 505HN Cryostat 

Light Microscopy

Nikon E600 Widefield Epifluorescence and Darkfield Microscopy System 
The Nikon E600 configuration consists of:

  • Excitation and emission filters for UV, green, red and far red dyes
  • Fiber optic darkfield illuminator for high-resolution darkfield imaging
  • Wide range of Plan and Plan-Fluor objectives ranging from 4X through 100X oil
  • Phase and DIC objectives and condensers
  • Micropublisher digital camera with Qimaging software


Location
The E600 system is located in the Building 1 B60
 


Nikon Optiphot Epifluorescence System 
The Nikon Optiphot Epifluorescence system configuration consists of:

  • Excitation and emission filters for red/green wide field fluorescence
    4,10,40 and 60X oil objectives
  • Zeiss Axiocam MRc digital camera and Axiovision software

Location
The Optiphot system moved to Azhar lab.
 

Nikon SMZ 1500 Stereomicroscope

The Nikon SMZ 1500 Stereomicroscope system consists of:

  • Filter sets for fluorescent imaging of EGFP and red ranges of dyes
  • Transmitted light imaging

Location
The Nikon SMZ 1500 system is located in the Building 1 B60.
 

Zeiss SteREO Lumar V.12 Stereomicroscope

The Zeiss Lumar V.12 system consists of:

  • Filter sets for fluorescent imaging of EGFP, Rhodamine, CFP, YFP, Cy3 and DAPI ranges of dyes
  • LCD cold light source for transmitted light imaging
  • Fiber optic illumination source
  • Zeiss Axiocam MRm digital camera with Axiovision software

Location
The Lumar V.12 system is located in Building 1 Room B59.

Website

Live Cell and Confocal Imaging Systems

Amira 5 Workstation 

Amira 6 Workstation 

Invitrogen EVOS FL Auto Cell Imaging System 

Leica SP8 Multiphoton Confocal 
The SP8 TCS multiphoton system is a state-of-the-art platform equipped with:

  • Upright DM6000 CFS microscope
  • 10X Dry, 20X Dry, 25X Water immersion, 40X Water, 63X Oil, and X-Clarity 25X objectives
  • Two internal PMTs
  • 488nm, 552nm, and 638nm lasers
  • Coherent Chameleon Ultra II 680-1080 tunable laser
  • HyD-RLD high sensitivity detector
  • LAS AF 3D analysis software

Location
The Leica SP8 system is located in Building 9 (Access through IRF personnel only for non-VA researchers)

IRF Contacts
Bob Price or Anna Harper

Website

 

Zeiss LSM 510 META Confocal Scanning Laser Microscope 
The Zeiss LSM 510 META CSLM is a state-of-the-art single photon spectral imaging system. The IRF system is equipped with:

 

  • UV, Argon, green HeNe and red HeNe lasers
  • 3 photomultiplier tubes
  • META detector
  • Transmitted light and DIC capability
  • Zeiss Axioplan 2 motorized microscope
  • Plan Neo 2.5X, 10X, 40X oil, PlanApo 20X and 63X oil, 40X water immersion objectives
  • Software packages for physiology, FRET, FRAP and 3D
  • Additional workstation with full software packages for offline image processing

Applications

  • Imaging of specimens labeled with multiple fluorochromes from the UV through far red ranges
  • Measurement of alterations in ion concentrations
  • Visualization of protein interactions
  • Determine protein kinetics
  • Quantitative co-localization studies
  • Excitation and emission fingerprinting of fluorochromes
  • Multichannel unmixing
  • 3D reconstructions


Location
The LSM 510 system is located in Building 1 Room B60.

Website 
 

Zeiss VivaTome Spinning Disk Confocal Live Cell Imaging System 
The Zeiss VivaTome system is a spinning disk confocal system mounted on a Zeiss Axiovert  200M inverted microscope stand. The system offers the ability to monitor rapidly occurring events within living cells without compromising resolution while employing high frequency low intensity illumination that substantially reduces photobleaching and phototoxicity. This in-vitro system allows for real-time observation and image recording of live cells by signal collected by phase contrast and fluorescence optics. The IRF system consists of:

Illumination Sources
High intensity HAL 100 source for brightfield and phase contrast imaging.
Exfo X-Cite 120 Metal Halide high intensity source providing wavelengths from 360-700nm at constant power across the illumination field.
Optics: Phase contrast and brightfield objectives:

10x NA 0.25 PH-1 Achroplan
20x NA 0.40 PH-2 Achroplan
40x NA 0.75 PH-2 Achroplan
Optics: Fluorescence objectives

10x NA 0.5 FLUAR
20x NA 0.75 FLUAR
40x oil NA 1.3 FLUAR
63x oil NA 1.4 Apochromatic
Filters and Dichroics

490/515 FITC, Cy2
555/570 TRITC,Cy3,Texas Red
635/670 Cy5
340-360/510 Fura-2 for ratiometric calcium imaging
492/520 for GFP imaging
360/460 for DAPI, Hoechst and AMCA imaging
Image Capture

Image capture is by a Hamamatsu ORCA-ER digital camera controlled by Kinetic Imaging Image Acquisition Software (AQM Advance-6)
Software Packages

Zeiss Vivatome image analysis package
Environmental Control
The environment of the microscope stage and specimen under observation is by a Zeiss M 200 Incubator chamber and heated stage and objective controllers. This system allows for the control and stabilization of the temperature, CO2 concentration and humidity.
Specimen holders

The microscope stage accommodates standard slides, Lab-Tek chambers, 35-60mm Petri Dishes, Microplates, and small, specialized culture chambers.
Applications

Long-term time-lapse studies including image capture in phase contrast for investigation of cell migration patterns and cell movements under normal and experimental conditions.
Monitoring of cell division.
Short term, high speed imaging (up to 30 frames/sec) for imaging of ion flux in living cells, in particular +calcium, sodium and pH.

Location
The Vivatome Confocal Live Cell Imaging System is located in Building 1 Room B59.

IRF Contact
Lorain Junor

Website  

Electron Microscopy Systems

JEOL 1400 Plus Transmission Electron Microscope 

The JEOL 1400 plus transmission electron microscope is a standard TEM system for imaging biological ultrastructure. The IRF system is equipped with:

  • Tungsten filament
  • Resolution of 0.2nm
  • Serial EM program for Tomography (+/- 60o tilt) and Stitching
  • Accelerating voltages adjustable to 120kV
  • Advanced Microscopy Techniques side mounted 8K bottom mount digital camera\

Applications

  • High resolution ultrastructural imaging
  • Immunocytochemistry with colloidal gold and other electron dense probes
  • Tomography
  • Diffraction

Location
The JEOL 1400 Plus system is located in Building 1 Room B59

IRF Contacts

Bob Price or Jeff Davis
 


JEOL JSM IT100  

The  scanning electron microscope is a fully integrated digital high vacuum system. The system in the IRF is equipped with:

  • Tungsten filiment
  • High resolution secondary and TV rate backscatter detectors
  • Resolution in secondary mode of 3.5nm
  • Resolution in backscattered mode of 10nm
  • Sample size of up to 5 inches in diameter can be fully imaged
  • Motorized X-Y stage that can be driven to stored positions
  • Preset Z working distances from 8, 15, 25, 39, and 48mm
  • Eucentric stage with tilt capablilty of -5 to + 90 at a working distance of 48mm
    360 rotation
  • Accelerating voltages from 0.5-30kV
  • Magnifications from 5X-300,000X
  • Visions Software Package
  • Polaroid film and digital image storage

Applications

  • Imaging surface morphology of biological and materials samples
  • Imaging cell: cell and cell: ECM interactions
  • Colloidal gold immunocytochemistry of surface molecules

Location

The JEOL JSM IT100 system is located in Building 1 Room B59 of the IRF.

 


Leica Ultracut R Ultramicrotome 


Polaron Sputter Coater 

Charged per run.


Reichert U3 Ultramicrotome descriptionpricing 



Tousimis Samdri-PVT-3B Critical Point Dryer 

Charged per run.

Image Analysis Software
 
Flow Cytometry/Cell Sorting

BD FACS Aria II
The FACS-Aria II high performance cell sorter is aflow cytometer capable of sorting upto 70,000 events per second while maintaining viability and function of cells in culture. This makes it an ideal system for sterile cell sorting for separating rare cell events, such as stem cells, dendritic cells, and NKT cells that often represents less than 1% to 2% of the total cells in a large population.

  • Solid state 488 and 640 lasers and sorting capabilities.
  • Automated Cell Deposition Unit and Aerosol Management system.
  • The 488 line has forward and side scatter and 4 fluorescent detectors. The 640 line has 2 fluorescent detectors.
  • Two and Four way sorting into 12X75mm tubes, 15 ml tubes, slides or culture plates.
  • Fluidics with 70, 85 and 100 micron nozzle tips.
  • Temperature control option for cooling of sort collection devices – tubes and plates
  • Optical filters for the 488 laser include FITC, PE, PE-Texas Red, PerCP, CY5.5 and PECY7.
  • Optical Filters for the 640 laser includes APC-CY7, Alexa700, APC
  • Computer and FACSDIVA software
    Printer

Applications

  • Analysis of cell surface antigens and biomarkers
  • Immunophenotyping
  • Cell size and granularity
  • Stem cells and hematopoietic cell lineage studies
  • Hematology
  • Apoptosis, live/dead cell assay
  • Cell cycle studies
  • Stem cell 4 way sorting
  • Cell viability and Functional assays
  • Rare cell population sorting
  • Slides and culture plate sorting

Location
The FACS Aria II is located in Building 1 Room B44 of the IRF

IRF Contact
Udai Singh

Website  
 

Beckman Coulter FC500 Flow Cytometer 
The Beckman Coulter FC-500 is aflow cytometer designed for qualitative and quantitative measurements of biological and physical properties of cells and fluorescent particles. This system is equipped with:

  • Argon ion 488 nm 20mW output laser
  • Red Helium-neon 633 nm 20mW output laser
  • Optical filters for 5 color analysis
  • Flow rates up to maximum 3300 events per second
  • 12X75 glass or plastic tubes
  • Single tube sampling mode
  • 32 tube Multi Carousel loader (designed for vortexing each tube individually).
  • Computer and CXP software
  • Printer

Applications

  • Analysis of cell surface antigens and biomarkers
  • Immunophenotyping
  • Cell size and granularity
  • Stem cell and hematopoietic cell lineage studies
  • Hematology
  • Apoptosis, live/dead cell assay
  • Cell cycle studies
  • Cell viability and Functional assays
  • DNA analysis

Location
The FC 500 is located in Building 1 Room B44 of the IRF

IRF Contact
Udai Singh

Website  

Flow Cytometer Workstation 

Molecular Analysis

AFP X-Ray Film Processor 

Automated X-ray film processing for Westerns etc.

4X4 inch (10X10cm) through 14X36 inch (35X91cm) film
Capable of processing up to 85 sheets per hour
Time and temperature adjustable for controlled development of film

Location
IRF Basement 

IRF Contact
Jeff Davis or Benny Davidson
 

BioRad BioPlex 
The BioPlex system is a multiplex analysis system that permits the simultaneous analysis of up to 100 different biomolecules (proteins, peptides, or nucleic acids) in a single microplate well. By multiplexing with the Bio-Plex system, researchers can dramatically increase the amount of useful information from rare or volume-limited samples such as mouse or rat serum, and decipher complex interrelationships among proteins involved in signal transduction pathways. The microplate platform allows the automated analysis of 96-well plates with a throughput of more than 1,800 assay points (for a 23–plex assay) in 30 minutes. The Bio-Plex suspension array is built around three core technologies:

The first is the family of fluorescently dyed microspheres, or beads, from Luminex Corp.
The second is a flow cytometer with two lasers and associated optics to measure biochemical reactions that occur on the surface of the microspheres.
The third is a high-speed digital signal processor to efficiently manage the fluorescent output.

Applications

  • Multiplex analysis of cytokines and phoshoproteins with prepared kits available from BioRad
  • Multiplex analysis of a variety of proteins by conjugating antibodies to beads via kits available from BioRad

Location
The BioPlex system is located in the Building 1 Room B60 lab of the IRF.

IRF Contact
Udai Singh 

Website 
 

BioRad BioPlex 200 System 
 

Location
Located on the VA Campus in building 9.

Website


BioRad GelDoc XR+ System and iMark Microplate Absorbance Reader 
Rapid system for imaging, documentation, and analysis of gels and blots for nucleic acids, protein gels, and blots. The iMark Microplate Absorbance Reader is an eight-channel, vertical path length photometer that measures the absorbance of the contents in the wells of a 96 well microtitration plate.  It an perform single or dual wavelength measurements and can report absorbance values to three decimal places.

Location
The GelDoc is located in the Building 1 Room B60 laboratory of the IRF

IRF Contact
Anna Harper/ Lorain Junor

Website
 

Illumina NextSeq 500 Sequencer 
The NextSeq 500 desktop sequencer is ideal for next generation sequencing (NGS).  With high and mid output flow cells, read lengths ranging from 2x 75 to 2x 150bp read are usually obtainable in less than 24 hours.  The instrument is capable of analyzing a broad range of sample types including 1-16 exomes, 1-20 transcriptomes, 6-96 targeted panels, or 12-40 gene expression profiling samples per run.

Applications

  • RNA sequencing
  • Targeted resequencing: Exome
  • Targeted resequencing: Amplicon
  • Whole-genome sequencing

Location
The NextGen 500 is located in Building 9 Rm 225

IRF Contact
Lorain Junor

Website


LiCor Odyssey CLX System 

Location
Located in Building 1 Room B59.

Website 


NanoPhotometer Pearl 
Micro-liter sample analysis of  nucleic acids   dsDNA, ssDNA, RNA, and Oligonucleotides, Labeling efficiency of proteins and protein dye, Bradford, Lowry, BCA, and Biuret applications

Location
The Agilent 2100 Bioanalyzer is located in the Building 1 Room B60 laboratory of the IRF

IRF Contact
Lorain Junor

Website  


QIAgility 
 The QIAgility and Rotor-Gene Q Real time PCR equipment provides compact bench top instruments that enable a rapid, high-precision PCR setup. The unmatched versatility of the QIAgility means that almost all tube and plate formats are supported, as well as Rotor-Discs for the Rotor-Gene Q. The QIAgility is operated via a computer with easy-to-use software.

Applications

  • Automated PCR setup in all formats
  • Convenient, easy-to-use software
  • Standardized results and increased productivity
  • Elimination of manual pipetting steps
  • Seamless integration with QIAGEN sample technologies
  • Outstanding thermal and optical performance due to rotary format
  • An unmatched optical range spanning UV to infrared wavelengths
  • High performance in multiple applications with QIAGEN assays

IRF Contact
Jay Potts

Location
Located on the VA Campus in building 9.

Website  
 

QIAsymphony System 
With a dedicated range of QIAsymphony Kits, the QIAsymphony SP enables sample preparation of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials. The QIAsymphony AS extends the capabilities of the QIAsymphony SP by integrating automated PCR assay setup, which, in combination with the Rotor-Gene Q and QIAGEN real-time and end-point PCR kits, enables you to optimize your PCR workflow and maximize your efficiency.

Applications

  • Purification of DNA and RNA from a wide range of samples
  • Automatic transfer of eluates to the QIAsymphony AS for assay setup
  • Continuous sample loading, with bar code reading for sample tracking
  • Import of sample lists and export of sample sheets

Website 

IRF Contact
Jay Potts

Location
Located on the VA Campus in building 9.

Website


Real Time PCR (BioRad CFX Connect) 

Location
The rtPCR CFX Connect is located in Building 1 Rm B59


Real Time PCR (BioRad iCycler IQ) 
Real time PCR, in conjunction with the appropriate fluorescent detection strategies,  allows the initial amount of nucleic acid in a reaction to be determined. Advances in the technology have led to significant improvements in the accuracy of DNA quantitation and gene expression analysis. Quantitation is achieved by measuring the increase in fluorescence during the exponential phase of the PCR reaction. The system in the IRF is equipped for:

Three color analysis for amplification of three dependant targets
Taqman, Sybr Green, primer based system and molecular beacon analyses
Multiple sample block formats for 0.2ml tubes, 96 well plates and 384 well PCR plates
Real time display of data acquisition

Applications

  • Quantitative comparison of genes of interest in housekeeping genes in a single sample
  • Quantitative detection of gene expression in genetically modified organisms
  • Analysis of single nucleotide polymorphisms (SNPs)
  • When used in conjunction with laser capture microdissection, real time PCR provides an excellent technique for comparison of genetic expression in specific cell types such as cancer vs. normal cells from a single region of tissue.

Location
The BioRad iCycler iQ system is located in the Building 1 Room B59 laboratory of the IRF

IRF Contact
Lorain Junor

Website


Real Time PCR (Qiagen Rotor-Gene Q) 

Location
Located on the VA Campus in building 9.

Website  


TECAN Freedom EVO System 
The TECAN Freedom robotic platform offers an advanced, proven and reliable liquid handling system for different scales and throughputs. The liquid handling and manipulative robotic arms provide an extendable work area and variable configurations. Freedom EVO workstations can automate a diverse range of applications including primary and secondary screening, cell growth and maintenance, nucleic acid sample preparation, hit picking, library management and sample weighing. Once the Freedom completes the specimen preparation the sample is automatically transferred to the integrated BioRad BioPlex 200 system for data acquisition and analysis.

Applications

  • Automated liquid handling for sample preparation and analysis
  • Multiplex analysis of cytokines and phosphoproteins with prepared kits available from BioRad
  • Multiplex analysis of a variety of proteins by conjugating antibodies to beads via kits available from BioRad

Location 
The TECAN BioPlex 200 systems are located in Building 9 (VA investigator and IRF service access only)

IRF Contact
Jay Potts

Website    

Other Equipment