Faculty and Staff
Kenneth B. Walsh, Ph.D.
|Title:||Associate Professor of Pharmacology, Physiology & Neuroscience
|Department:||Pharmacology, Physiology and Neuroscience
School of Medicine
Pharmacology, Physiology & Neuro
University of Rochester, School of Medicine
University of Cincinnati, College of Medicine
Work in my laboratory involves the development of cellular assays for studying biologically active compounds such as natural products, drugs and hormones. Delta-9-tetrahydrocannibinol (THC) and cannabidiol (CBD) are the two major cannabinoids found in marijuana (cannabis sativa). To date, investigators have isolated over one hundred chemically distinct cannabinoids from marijuana. It is anticipated that these cannabinoids and related compounds will be useful for treating anxiety, pain, inflammation and other disorders.
The figure to the right shows the structures of THC (top) and CBD (bottom).
In one project, immortalized neuronal cell lines are stably transfected with cannabinoid CB1 receptors using recombinant lentiviral vectors. The effects of THC and CBD on the activation of G protein-gated inward rectifier K+ (GIRK) channels are then determined using a membrane potential-sensitive fluorescent dye assay. These results are compared with the ability of the cannabinoids to inhibit adenylyl cyclase and to stimulate the recruitment of β-arrestin to the plasma membrane.
- Walsh KB. Targeting cardiac K+ channels for state-of-the-art drug discovery. Expert Opinion in Drug Discovery 2015; 10:157-169.
- Li H, Zhu Y, Islam S, Rahman MA, Walsh KB, Koley G. Graphene field effect transistors for highly sensitive and selective detection of potassium ions. Sensors and Actuators B: Chemical 2017; 253:759-765.
- Walsh KB, Gay EA, Blough BE, Geurkink DW. N-(2-methoxyphenyl) benzenesulfonamide, a novel regulator of neuronal G protein-gated inward rectifier K+ channels. European Journal of Pharmacology 2017; 815:233-240
- Anderson H, Piroli G, Walsh KB. A real time screening assay for cannabinoid CB1 receptor-mediated signalling. Journal of Pharmacological and Toxicological Methods 2018;94-44-49
- Walsh KB, Li H, Koley G. Graphene alters the properties of voltage-gated Ca2+ channels in H9c2 cardiomyocytes. Biomedical Physics and Engineering Express 2018; 4:065004.