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F. Wayne Outten Group Site


F. Wayne Outten Lab publications at USC

Reverse chronological order (most recent to oldest)


All publications

A complete list of all publications from F. Wayne Outten in PubMed, with some articles available free through HHS Public Access (PMC).

2020 jbc

Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues.

Blahut M, Wise CE, Bruno MR, Dong G, Makris TM, Frantom PA, Dunkle JA, Outten FW. J Biol Chem.  2019.  294(33):  12444-12458.

2019 biochem

Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS.

Dunkle JA, Bruno MR, Outten FW, Frantom PA. Biochemistry.  2019.  58(6):  687-696.

jbc 2019

Evidence that a respiratory shield in Escherichia coli protects a low-molecular-mass FeII pool from O2-dependent oxidation.

Wofford JD, Bolaji N, Dziuba N, Outten FW, Lindahl PA.  J Biol Chem.  2019.  294(1):  50-62.

2019 RB

Extra-mitochondrial Cu/Zn superoxide dismutase (Sod1) is dispensable for protection against oxidative stress but mediates peroxide signaling in Saccharomyces cerevisiae

Montllor-Albalate C, Colin AE, Chandrasekharan B, Bolaji N, Andersen JL, Wayne Outten F, Reddi AR.  Redox Biol.   2019.  21: 101064

MO cover

Nickel exposure reduces enterobactin production in Escherichia coli

Washington-Hughes CL, Ford GT, Jones AD, McRae K, Outten FW.  Microbiology Open.  2019.  8(4): e00691


Changes in protein dynamics in Escherichia coli SufS reveal a possible conserved regulatory mechanism in Type II Cysteine Desulfurase systems.

Kim D, Singh H, Dai Y, Dong G, Busenlehner LS, Outten FW, Frantom PA.  Biochemistry.  2018.  57(35): 5210-5217.


Conserved cysteine residues are necessary for nickel-induced allosteric regulation of the metalloregulatory protein YqjI (NfeR) in E. coli.

Blahut M, Dzul S, Wang S, Kandegedara A, Grossoehme NE, Stemmler T, Outten FW.   J Inorg Biochem. 2018.  184: 123-133.  


Heme dynamics and trafficking factors revealed by genetically encoded fluorescent heme sensors.

Hanna DA, Harvey RM, Martinez-Guzman O, Yuan X, Chandrasekharan B, Raju G, Outten FW, Hamza I, Reddi AR.  Proc Natl Acad Sci U S A. 2016. 113(27): 7539-7544.


Functional dynamics revealed by the structure of the SufBCD complex, a novel ATP-binding cassette (ABC) protein that serves as a scaffold for iron-sulfur cluster biogenesis.

Hirabayashi K, Yuda E, Tanaka N, Katayama S, Iwasaki K, Matsumoto T, Kurisu G, Outten FW, Fukuyama K, Takahashi Y, Wada K.   J Biol Chem. 2015.  290(50): 29717-29731.


SufE D74R substitution alters active site loop dynamics to further enhance SufE interaction with the SufS cysteine desulfurase.

Dai Y, Kim D, Dong G, Busenlehner LS, Frantom PA, Outten FW.  Biochemistry. 2015.  54(31): 4824-4833.  


Recent advances in the Suf Fe-S cluster biogenesis pathway: Beyond the Proteobacteria.

Outten FW.  Biochim Biophys Acta. 2015.  1853(6): 1464-1469  


Interplay between oxygen and Fe-S cluster biogenesis: insights from the Suf pathway.

Boyd ES, Thomas KM, Dai Y, Boyd JM, Outten FW.  Biochemistry.  2014.  53(37): 5834-5847. 


Communication between binding sites is required for YqjI regulation of target promoters within the yqjH-yqjI intergenic region.

Wang S, Blahut M, Wu Y, Philipkosky KE, Outten FW.  J Bacteriol.  2014. 196(17): 3199-3207. 


A stress-responsive Fe-S cluster biogenesis system in bacteria – the suf operon of Gammaproteobacteria.

Outten, F.W.  2014.  In T. Rouault (Ed.), Iron-Sulfur Clusters in Chemistry and Biology, Berlin, Boston: De Gruyter. 


Escherichia coli SufE sulfur transfer protein modulates the SufS cysteine desulfurase through allosteric conformational dynamics.

Singh H, Dai Y, Outten FW, Busenlehner LS.  J Biol Chem.  2013.  288(51): 36189-200. 


Lability and liability of endogenous copper pools.

Outten FW, Munson GP. J Bacteriol.  2013.  195(20): 4553-5. 


Fe-S cluster biogenesis in Archaea and Bacteria.

Chahal, H.K., Boyd, J., and Outten F.W.  2012.   In V. Culotta and R. A. Scott (Eds.), Encyclopedia of Inorganic and Bioinorganic Chemistry: Metals in Cells, West Sussex, UK: John Wiley and Sons, Ltd. 


The E. coli SufS-SufE sulfur transfer system is more resistant to oxidative stress than IscS-IscU.

Dai Y, Outten FW.  FEBS Lett.  2012.  586(22):4016-22.  


Separate FeS scaffold and carrier functions for SufB₂C₂ and SufA during in vitro maturation of [2Fe2S] Fdx.

Chahal HK, Outten FW.  J Inorg Biochem. 2012.  116: 126-34.


Fur and the novel regulator YqjI control transcription of the ferric reductase gene yqjH in Escherichia coli.

Wang S, Wu Y, Outten FW.  J Bacteriol.  2011.  193(2): 563-74. 


SufD and SufC ATPase activity are required for iron acquisition during in vivo Fe-S cluster formation on SufB.

Saini A, Mapolelo DT, Chahal HK, Johnson MK, Outten FW.  Biochemistry. 2010.  49(43): 9402-12.


The SufBCD Fe-S scaffold complex interacts with SufA for Fe-S cluster transfer.

Chahal HK, Dai Y, Saini A, Ayala-Castro C, Outten FW.  Biochemistry.  2009.  48(44): 10644-53.


Native Escherichia coli SufA, coexpressed with SufBCDSE, purifies as a [2Fe-2S] protein and acts as an Fe-S transporter to Fe-S target enzymes.

Gupta V, Sendra M, Naik SG, Chahal HK, Huynh BH, Outten FW, Fontecave M, Ollagnier de Choudens S.  J Am Chem Soc.  2009.  131(17): 6149-53. 


Molecular dynamism of Fe-S cluster biosynthesis implicated by the structure of the SufC2-SufD2 complex.

Wada K, Sumi N, Nagai R, Iwasaki K, Sato T, Suzuki K, Hasegawa Y, Kitaoka S, Minami Y, Outten FW, Takahashi Y, Fukuyama K.  J Mol Biol.  2009.  387(1): 245-58. 


IscR controls iron-dependent biofilm formation in Escherichia coli by regulating type I fimbria expression.

Wu Y, Outten FW.  J Bacteriol.  2009. 191(4): 1248-57. 


Iron-based redox switches in biology.

Outten FW, Theil EC.  Antioxid Redox Signal.  2009.  11(5): 1029-46.  


The impact of O2 on the Fe-S cluster biogenesis requirements of Escherichia coli FNR.

Mettert EL, Outten FW, Wanta B, Kiley PJ.  J Mol Biol.  2008.  384(4): 798-811. 


Fe-S cluster assembly pathways in bacteria.

Ayala-Castro C, Saini A, Outten FW.  Microbiol Mol Biol Rev.  2008.  72(1): 110-25.


Iron-sulfur clusters as oxygen-responsive molecular switches.

Outten FW.  Nat Chem Biol.  2007.  3(4): 206-7. 


SufE transfers sulfur from SufS to SufB for iron-sulfur cluster assembly.

Layer G, Gaddam SA, Ayala-Castro CN, Ollagnier-de Choudens S, Lascoux D, Fontecave M, Outten FW.  J Biol Chem.  2007. 282(18): 13342-50. 

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