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School of Medicine Columbia

Light Microscopy

Light microscopy, a cornerstone of scientific exploration, employs visible light to magnify and visualize specimens at the cellular and subcellular levels. By harnessing the principles of optics, this technique allows researchers to uncover the intricacies of biological structures with remarkable clarity and detail, paving the way for breakthroughs in fields ranging from biology and medicine to materials science.

Equipment Options

Details

  • Excitation and emission filters for UV, green, red and far red dyes
  • Fiber optic darkfield illuminator for high-resolution darkfield imaging
  • Wide range of Plan and Plan-Fluor objectives ranging from 4X through 100X oil
  • Phase and DIC objectives and condensers
  • Micropublisher digital camera with Qimaging software

Location

IRF B59

IRF Contacts

Anna Harper

The Nikon SMZ1500 Microscope, Model C-DSD115, is an advanced imaging system designed for researchers and scientists who demand high-quality, high-resolution imaging capabilities. This microscope features a range of advanced technologies to provide exceptional imaging quality and versatility.

The SMZ1500 microscope utilizes a zoom system with a 15:1 zoom ratio, which provides a magnification range of 0.75x to 11.25x. This allows for precise imaging of a wide range of specimens, from small cells to large tissue samples. The microscope also features an advanced optical system with Nikon’s exclusive CFI60 infinity optics, which provides exceptional resolution, clarity, and contrast.

Location

IRF B59

IRF Contact

Lorain Junor

 

The Discover Echo Revolution is the world’s first and only hybrid, 4-in-1 microscope. Options include:

  • The upright microscope- ideal for glass slides
  • The inverted microscope- ideal for live cell imaging
  • Transmitted light- brightfield, darkfield, phase contrast, and Polarized light
  • Fluorescence- automated LED illumination and one touch image overlay

Using the large touchscreen computer, users can control camera and illumination functions with their fingertip.

Widefield Fluorescence Images can encounter haze artifacts produced by out of focus light. ECHO’s proprietary Digital Haze Reduction (DHR) software module reveals fine details by suppressing noise and reducing blur to provide stunning, crystal-clear images

Location

IRF B59

IRF Contacts

Anna Harper

The EVOS is a fully automated, digital inverted multi-channel fluorescence and transmitted light imaging system equipped with: 

  • Light cubes: Dapi, GFP, Texas Red and Cy5
  • 4X, 10X phase, 20X, 20X phase, 40X and 60X objectives
  • Vessel holders available: double slide, multiwell, 35mm dish, 60mm dish, 100mm dish, T-25 flask and T-75 flask

Location

IRF B59

IRF Contacts

Lorain Junor or Anna Harper

Equipment Website

With the new Power HyD S detectors, the STELLARIS 5 sets a new standard for the quality of images and quantity of information generated. This perfected imaging performance is easily attainable thanks to the smart user interface, ImageCompass, which guides you through your experiment set up and acquisition in an easy and intuitive manner.

Details

  • UV, 5 Fixed Diode lasers 405nm, 448nm, 488nm, 514nm, 561nm, 638nm
  • HyD S detectors
  • Transmitted light 
  • 10X, 20X, 25X water, 40X oil, 63X oil objectives
  • Software package includes LASX 3D
  • Lightning Super Resolution Capabilities

Location

IRF B60

IRF Contacts

Anna Harper

The Leica Stellaris 8 FALCON is an inverted microscope offering enhanced detection efficiency with STED technology.  Capable of live cell STED imaging throughout the visible spectrum.  The extended detection range up to 850nm plus the expanded range in the visible from 440nm to 790nm allow the application and separation of an extended range of spectrally overlapping fluorophores, up to 5 simultaneously. 

Details:

  • STED white light laser
  • UV, 5 Fixed Diode lasers 405nm, 448nm, 488nm, 514nm, 561nm, 638nm
  • HyD S & X detectors
  • Transmitted light
  • TauSense and TauSTED 3D technology
  •  Lightning Super Resolution Capabilities
  • HC PL FLUOTAR 5x/0.15 CS2
  • HC PL APO 20x/0.75 CS2
  • HC PL APO 40x/1.10 W CORR CS2
  • HC PL APO 63x/1.40 OIL CS2
  • HC PL APO 86x/1.20 W motCORR STED W
  • HC PL APO 100x STED

Location

Coker Life Sciences (CLS) Room 711

IRF Contacts

Anna Harper

Light sheet fluorescence microscopy (LSFM) is ideal for fast and gentle imaging of whole living model organisms, tissues and cells as they develop – over extended periods of time. What’s more, use ZEISS Lightsheet 7 to image large optically cleared specimens in toto – with subcellular resolution. Dedicated optics, sample chambers and holders allow adaptation to the refractive index of your chosen clearing method.

Location

IRF B59

IRF Contacts

Anna Harper

 


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