Faculty and Staff
|Title:||Assistant Professor of Pathology, Microbiology & Immunology
|Department:||Pathology, Microbiology & Immunology
School of Medicine
Pathology, Microbiology & Immunology
Graduate Studies: University of Texas at Austin, Austin, TX
Postdoctoral: National Institutes of Health, Bethesda, MD
Faculty: Virginia Commonwealth University, Richmond, VA
My research is in the field of allergy and asthma. The goal is to identify and characterize the molecular and immunological mechanisms that regulate mast cells, key effector cells of IgE-mediated immediate hypersensitivity reactions. The current focus is to (1) Determine the role of extracellular adenosine in the regulation of mediator release from human mast cells; and (2) Determine the effect of Resveratrol on IgE-dependent mast cell function and the allergic response.
The release of allergic/inflammatory mediators from mast cells can occur following stimulation with a variety of agents. However, allergic responses are more closely associated with cross-linking of the high affinity Fc receptor for IgE, FcεRI, by multivalent antigen, which results in the immediate release of pre-formed inflammatory mediators stored within cytoplasmic granules (degranulation), biosynthesis and release of lipid mediators, and secretion of de-novo produced cytokines. Adenosine is a purine nucleoside produced from the de-phosphorylation of ATP; thus, its production is intimately connected to cellular metabolism. Our interest in adenosine stems from its ability to potentiate and inhibit mast cell degranulation, and to induce bronchoconstriction in asthmatics. We recently identified a previously unrecognized differential degranulation response to adenosine by human lung and skin mast cells, and identified human A3AR as a potentiating receptor. Current studies are aimed at (1) Defining a role for intracellular adenosine as a negative regulator of mast cell degranulation, (2) Identifying a role for IgE in the regulation of adenosine receptor gene expression, and (3) Determining the effect of adenosine on IgE-induced activation of the NF-κB pathway, and identifying the adenosine receptor(s) responsible.
- Gomez G, Sitkovsky MV. Differential requirement for A2a and A3 adenosine receptors for the protective effect of inosine in vivo. Blood. Dec 15; 102(13): 4472-8. 2003.
- Odom S*, Gomez G*, Kovarova M, Furumoto Y, Ryan JJ, Wright HV, Gonzales-Espinosa C, Hibbs ML, Harder KW, Rivera J. Negative regulation of Immunoglobulin E-dependent allergic responses by Lyn kinase. J Exp Med. Jun 7; 199(11): 1491-502. 2004. *co-first authors
- Gregorio Gomez, Carlos D. Ramirez, Juan Rivera, Manish Patel, Farnaz Norozian, Harry V. Wright, Mohit V. Kashyap, Lawrence B. Schwartz, Christopher L. Kepley, and John J. Ryan. TGF1 inhibits mast cell FcεRI expression. J Immunol. 174: 5897-5993. 2005.
- Gregorio Gomez, Claudia Gonzalez-Espinosa, Sandra Odom, Gabriela Baez, M. Eugenia Cid, John J. Ryan, and Juan Rivera. Impaired FcεRI-dependent gene expression and defective eicosanoid and cytokine production as a consequence of Fyn-deficiency in mast cells. J. Immunol. 175(11):7602-7610. 2005.
- John J. Ryan, Henry R. Bateman, Alex Stover, Greg Gomez, Sarah K. Norton, Wei Zhao, Lawrence B. Schwartz, Robert Lenk, and Christopher L. Kepley. Fullerene Nanomaterials Inhibit the Allergic Response. J. Immunol. 179:665-672. 2007.
- Gomez G, Jogie-Brahim S, Shima M, Schwartz LB. Omalizumab Reverses the Phenotypic and Functional Effects of IgE-Enhanced FcεRI on Human Skin Mast Cells. J Immunol. 2007 Jul 15; 179(2):1353-61. PMC2396781.
- Gregorio Gomez, Lawrence Schwartz and Christopher L. Kepley. Syk deficiency in human non-releaser lung mast cells. Clin Immunol. 125: 112-115. 2007. PMC2174611.
- Wei Zhao, Gregorio Gomez, Shao-Hua Yu, John J. Ryan and Lawrence B. Schwartz. TGF-b1 Attenuates Mediator Release and De Novo Kit Expression by Human Skin Mast Cells through a Smad-dependent Pathway. J. Immunol. 181(10): 7263-72. 2008. PMC2645416.
- Gregorio Gomez, Wei Zhao and Lawrence B. Schwartz. Disparity in FcεRI-induced degranulation of primary human lung and skin mast cells exposed to adenosine. J Clin Immunol. 2011 Jun; 31(3):479-87. Epub 2011 Mar 25. PMID: 21437670, PMC3417298.
- Wei Zhao, Gregorio Gomez, Matthew R. Macey, Christopher L. Kepley, Lawrence B. Schwartz. In vitro desensitization of human skin mast cells. J. Clin Immunol. 2012 Feb; 32(1):150-160. Epub. Oct. 19, 2011. PMID: 22009002, PMC pending.