Faculty and Staff
|Title:||Associate Professor of Cell Biology & Anatomy
|Department:||Cell Biology and Anatomy
School of Medicine
|Office:||Basic Science Bldg 1|
|Resources:||Lab Phone: 803-216-3833|
1989 The 4th Military Medical University – M.D. – P.R. China
2001 Ehime University School of Medicine – Ph.D. – Molecular Medicine
The majority of cardiovascular disease results from complications of vascular disorders, e.g., atherosclerosis that becomes worse under the circumstance with obesity and diabetes. Dr. Cui has a research interest in vascular biology with special reference to the vasculopathy associated with obesity and diabetes. Dr. Cui’s laboratory takes a multi-disciplinary approach to address the molecular and cellular mechanism that links obesity, diabetes and vascular disease, with a specific focus on the role of ubiquitin-proteasome system in the maintenance of vascular homeostasis.
There are four areas of technical strengths in Dr. Cui’s laboratory: 1) molecular
biology including most of the routine DNA, RNA and protein techniques; 2) cellular
biology including primary culture of vascular smooth muscle cells, endothelial cells,
macrophages and adipocytes, dissection of signal transduction pathways involved in
cellular metabolism, migration and proliferation as well as inflammatory responses;
3) development and characterization of animal models of vascular disease through transgenic
approaches; 4) vascular physiology analyses in cell, organ and whole animal levels.
Two major projects currently underway include:
- Defining mechanism that regulates Keap1-mediated Nrf2 ubiquitination, degradation, anti-oxidative gene expression in vasculature.
- Determining mechanism that controls the deubiquitinating enzymes such as UCH-L1- or CYLD-mediated suppression of inflammatory responses in vasculature.
- Bai Y, Cui W, Xin Y, Miao X, Barati MT, Zhang C, Chen Q, Tan Y, Cui T, Zheng Y, Cai L (2013) Prevention by sulforaphane of diabetic cardiomyopathy is associated with up-regulation of Nrf2 expression and transcription activation. J Mol Cell Cardiol 57:82-95.
- Wang Y, Sun W, Du B, Miao X, Bai Y, Xin Y, Tan Y, Cui W, Liu B, Cui T, Epstein PN, Fu Y, Cai L (2013) Therapeutic effect of MG-132 on diabetic cardiomyopathy is associated with its suppression of proteasomal activities: roles of Nrf2 and NF-κB. Am J Physiol Heart Circ Physiol 304(4):H567-578.
- Xing Y, Niu T, Wang W, Li J, Li S, Janicki JS, Ruiz S, Meyer CJ, Wang XL, Tang D, Zhao Y, Cui T (2012) Triterpenoid dihydro-CDDO-trifluoroethyl amide protects against maladaptive cardiac remodeling and dysfunction in mice: a critical role of Nrf2. PLoS One 7:e44899.
- Tan Y, Ichikawa T, Li J, Si Q, Yang H, Chen X, Goldblatt CS, Meyer CJ, Li X, Cai L, Cui T(2011) Diabetic downregulation of Nrf2 activity via ERK contributes to oxidative stress-induced insulin resistance in cardiac cells in vitro and in vivo. Diabetes 60:625-633.
- Li J, Zhang C, Xing Y, Janicki JS, Yamamoto M, Wang XL, Tang DQ, Cui T (2011) Up-regulation of p27(kip1) contributes to Nrf2-mediated protection against angiotensin II-induced cardiac hypertrophy. Cardiovasc Res 90:315-324.
- Li J, Zhang C, Xing Y, Janicki JS, Yamamoto M, Wang XL, Tang DQ, Cui T*. Up-regulation of p27kip1 contributes to Nrf2-mediated protection against angiotensin II-induced cardiac hypertrophy. Cardiovasc Res. 2011 May 1;90(2):315-24
- Tan Y, Ichikawa T, Li J, Si Q, Yang H, Chen X, Goldblatt CS, Meyer CJ, Cai L, Cui T*. Diabetic down-regulation of Nrf2 activity via ERK contributes to oxidative stress-induced insulin resistance in cardiac cells in vitro and in vivo. Diabetes. 2011 Feb;60(2):625-33
- Ichikawa T, Li J, Dong X, Potts JD, Tang DQ, Li DS, Cui T*. Ubiquitin carboxyl terminal hydrolase L1 negatively regulates TNF-mediated vascular smooth muscle cell proliferation via suppressing ERK activation. Biochem Biophys Res Commun. 2010 Jan 1;391(1):852-6
- Li J, Ichikawa T, Jin Y, Hofseth LJ, Nagarkatti P, Nagarkatti M, Windust A, Cui T*. An essential role of Nrf2 in Americang ginseng-mediated anti-oxidative actions in cardiomyocytes. J Ethnopharmacol. 2010 Jul 20; 130(2):222-230.
- Ichikawa T, Li J, Nagarkatti P, Nagarkatti M, Hofseth LJ, Windust A, Cui T*. American ginseng preferentially suppresses STAT/iNOS signaling in activated macrophages. J Ethnopharmacol. 2009; 17; 125(1):145-50
- Cui TG, Ichikawa T, Yang M, Dong X, Li J, Cui T*. An emerging role of deubiquitinating enzyme Cylindromatosis (CYLD) in the tubulointerstitial inflammation of IgA nephropathy. Biochem Biophys Res Commun. 2009; 11; 390(2):307-12
- Li J, Ichikawa T, Janicki JS, Cui T*. Targeting the Nrf2 pathway against cardiovascular disease. Expert Opin Ther Targets. 2009; 13(7):785-94
- Ichikawa T, Li J, Meyer CJ, Janicki JS, Hannink M, Cui T*. Dihydro-CDDO-trifluoroethyl amide (dh404), a novel Nrf2 activator, suppresses oxidative stress in cardiomyocytes. PLoS One.2009 Dec 21;4(12):e8391
- Li J, Ichikawa T, Villacorta L, Janicki JS, Brower G, Yamamoto M, Cui T*. Nrf2 protect against maladaptive cardiac responses to hemodynamic stress. Arterioscler Thromb Vasc Biol. 2009; 29(11):1843-50
- Ichikawa T, Zhang J, Chen K, Liu Y, Schopfer FJ, Baker PRS, Freeman BA, Chen YE, Cui T*. Nitroalkenes suppress LPS-induced STAT signaling in macrophages: A critical role of MKP-1. Endocrinology 2008, 149(8):4086-4094
- Villacorta L, Zhang J, Schopfer FJ, Baker PRS, Freeman BA, Chen YE, Cui T*. Nitrated Linoleic acids inhibit proliferation of vascular smooth muscle cells via Keap1/Nrf2 pathways. Am J Physiol. 2007, 293(1):H770-6.